Ultrasonic Extracts
نویسندگان
چکیده
Assay conditions have been established which allow maximum expression of phosphodeoxyribomutase activity in ultrasonic extracts of Escherichia coil K-12. The enzyme requires 2-mercaptoethanol, manganous ions, and glucose 1, 6-diphosphate for optimal activity. When cells are grown in minimal medium with glycerol as the carbon source and supplemented with 10-' M thymidine, phosphodeoxyribomutase is induced threeto four-fold. Cell pellets may be frozen for 24 hr before sonic disruption without loss of enzyme activity. The assay is highly reproducible.
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